Evaluation of MgO-ZnO-Crab Shell Biofillers as Reinforcement for Biodegradable Polylactic Acid (PLA) Composite

Authors

  • A. O. Ogunsanya Bells University of Technology
  • E. B. Iorkohol Bells University of Technology
  • D. Arinze Bells University of Technology
  • O. Ogundoyin University of Lagos

Keywords:

Bone Fracture, degradation, hydroxyapatite, stability, hygroscopic characteristics, porosity

Abstract

Biodegradable polyester obtained from renewable, eco-friendly materials, and natural additives made from debris of production of seafood to create biocomposites is nowadays a possibility. This paper evaluates the physical, morphological, and chemical properties and the degradation stability of polylactic acid/biofillers (magnesium oxide/zinc oxide/crab shell particles) composite as a viable biocomposite material in bone engineering applications. The biofiller showed hygroscopic characteristics. Surface morphology of the composite showed fractured surfaces with interconnected pores suitable for bone cells’ implantation enhancement and propagation. Biofillers effect accelerates the precipitation of calcium apatite formation after 28 days of immersion. The XRD spectra confirmed high composite crystallinity structure of 93.4% due to the nucleation effects of the biofillers. The beneficial role of reinforcing polylactic acid polymer with biofiller showed average pH value of 7.36 and apparent porosity of 40%. Findings from this paper have revealed that the use of crab shell debris such as crab shell can become a resource in biocomposite fabrication. The addition of biofillers provided an effective reinforcement in polylactic acid polymer matrix and hence contributed towards sustainable developments of natural resource materials and biodegradable and bioresorbable material without polluting the environment.

References

Aldana, A. A. and Abraham, G. A. (2017). Current advances in electrospun gelatin-based scaffolds for tissue engineering applications. International Journal of Pharmaceutics, 523(2), 441–453. https://doi.org/10.1016/j.ijpharm.2016.09.044

Antoniac, I.; M. Miculescu; V. Mănescu; A. Stere; P. H. Quan; G. Păltânea; A. Robu and K. Earar. (2022). Magnesium-based alloys used in orthopedic surgery. Materials, 15(3), 1148. https://doi.org/10.3390/ma15031148

Athanasoulia, I. G. I.; M. N. Christoforidis; D. M. Korres and P. A. Tarantili. (2017). The effect of hydroxyapatite nanoparticles on crystallization and thermomechanical properties of PLLA matrix. Pure and Applied Chemistry, 89(1), 125–140. https://doi.org/10.1515/pac-2016-0912

Bajpai, A.; J. Bajpai; R. K. Saini; P. Agrawal and A. Tiwari. (2016). Smart biomaterial devices: Polymers in biomedical sciences. CRC Press.

Balakrishnan, G.; R. Velavan; K. M. Batoo and E. H. Raslan. (2020). Microstructure, optical and photocatalytic properties of MgO nanoparticles. Results in Physics, 16, 103013. https://doi.org/10.1016/j.rinp.2020.103013

Dauda, K.; C. Majebi; A. Ayoola; O. Agboola; A. Popoola; O. Fayomi; S. Banjo; J. Sojobi and A. Ogunsanya. (2021). Biosmart materials and its innovative characteristics in protective composite coating application. 1107(1), 012216. https://doi.org/10.1088/1757-899X/1107/1/012216

Farshchi, N. and Ostad, Y. K. (2020). Sepiolite as a nanofiller to improve mechanical and thermal behavior of recycled high-density polyethylene. Progress in Rubber, Plastics and Recycling Technology, 36(3), 185–195. https://doi.org/10.1177/1477760620918596

Fattahi, F.; A. Khoddami and O. Avinc. (2019). Poly (lactic acid)(PLA) nanofibers for bone tissue engineering. Journal of Textiles and Polymers, 7(2), 47–64.

Funabashi, M.; F. Ninomiya; E. D. Flores and M. Kunioka. (2010). Biomass carbon ratio of polymer composites measured by accelerator mass spectrometry. Journal of Polymers and the Environment, 18, 85–93. https://doi.org/10.1007/s10924-010-0166-3

Gao, C.; S. Peng; P. Feng and C. Shuai (2017). Bone biomaterials and interactions with stem cells. Bone Research, 5(1), 1–33. https://doi.org/10.1038/boneres.2017.59

Gigante, V.; P. Cinelli; M. C. Righetti; M. Sandroni; L. Tognotti; M. Seggiani and A. Lazzeri. (2020). Evaluation of mussel shells powder as reinforcement for PLA-based biocomposites. International Journal of Molecular Sciences, 21(15), 5364. https://doi.org/10.3390/ijms21155364

Gortsas, T. V.; S. Tsinopoulos; E. Polyzos; L. Pyl; D. Fotiadis and D. Polyzos. (2022). BEM evaluation of surface octahedral strains and internal strain gradients in 3D-printed scaffolds used for bone tissue regeneration. Journal of the Mechanical Behavior of Biomedical Materials, 125, 104919. https://doi.org/10.1016/j.jmbbm.2021.104919

Gritsch, L.; M. Maqbool; V. Mouriño; F. E. Ciraldo; M. Cresswell; P. R. Jackson; C. Lovell and A. R. Boccaccini. (2019). Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: Copper and strontium. Journal of Materials Chemistry B, 7(40), 6109–6124. https://doi.org/10.1039/C9TB00897G

Hamester, M. R. R.; P. S. Balzer and D. Becker (2012). Characterization of calcium carbonate obtained from oyster and mussel shells and incorporation in polypropylene. Materials Research, 15(2), 204–208. https://doi.org/10.1590/S1516-14392012005000014

Iqbal, N.; T. M. Braxton; A. Anastasiou; E. M. Raif; C. K. Y. Chung; S. Kumar; P. V. Giannoudis and A. Jha. (2022). Dicalcium Phosphate Dihydrate Mineral Loaded Freeze-Dried Scaffolds for Potential Synthetic Bone Applications. Materials, 15(18), 6245. https://doi.org/10.3390/ma15186245

Jahani, B.; K. Meesterb; X. Wanga and A. Brooksc. (2020). Biodegradable Magnesium-Based alloys for bone repair applications: Prospects and challenges. Biomed Sci Instrum, 56(2), 292–304.

Jayaramudu, J.; K. Das; M. Sonakshi; G. S. M. Reddy; B. Aderibigbe; R. Sadiku and S. S. Ray. (2014). Structure and properties of highly toughened biodegradable polylactide/ZnO biocomposite films. International Journal of Biological Macromolecules, 64, 428–434. https://doi.org/10.1016/j.ijbiomac.2013.12.034

Kim, I.; K. Viswanathan; G. Kasi; K. Sadeghi; S. Thanakkasaranee and J. Seo. (2019). Poly (lactic acid)/ZnO bionanocomposite films with positively charged ZnO as potential antimicrobial food packaging materials. Polymers, 11(9), 1427. https://doi.org/10.3390/polym11091427

Kunitake, M. E.; L. M. Mangano; J. M. Peloquin; S. P. Baker and L. A. Estroff. (2013). Evaluation of strengthening mechanisms in calcite single crystals from mollusk shells. Acta Biomaterialia, 9(2), 5353–5359. https://doi.org/10.1016/j.actbio.2012.09.030

Levengood, S. K. L. and Zhang, M. (2014). Chitosan-based scaffolds for bone tissue engineering. Journal of Materials Chemistry B, 2(21), 3161–3184. https://doi.org/DOI https://doi.org/10.1039/C4TB00027G

Li, H. Y.; Y. Q. Tan; L. Zhang; Y. X. Zhang; Y. H. Song; Y. Ye and M. S. Xia. (2012). Bio-filler from waste shellfish shell: Preparation, characterization, and its effect on the mechanical properties on polypropylene composites. Journal of Hazardous Materials, 217–218, 256–262. https://doi.org/10.1016/j.jhazmat.2012.03.028

Li, X.; W. Yu; L. Han; C. Chu; J. Bai and F. Xue (2019). Degradation behaviors of Mg alloy wires/PLA composite in the consistent and staged dynamic environments. Materials Science and Engineering: C, 103, 109765. https://doi.org/10.1016/j.msec.2019.109765

Monia, T. (2022). β-TCP/DCPD-PHBV (40%/60%): Biomaterial made from bioceramic and biopolymer for bone regeneration; investigation of intrinsic properties. Journal of Applied Biomaterials & Functional Materials, 20, 22808000221088950. https://doi.org/10.1177/22808000221088950

Morsi, M. A. and Abd Elhamid, M. H. (2019). Effect of iron doped hydroxyapatite nanoparticles on the structural, morphological, mechanical and magnetic properties of polylactic acid polymer. Journal of Materials Research and Technology, 8(2), 2098–2106. https://doi.org/10.1016/j.jmrt.2019.01.017

Offner, D.; G. F. de Grado; I. Meisels; L. Pijnenburg; F. Fioretti; N. Benkirane-Jessel and A. M. Musset. (2019). Bone grafts, bone substitutes and regenerative medicine acceptance for the management of bone defects among French population: Issues about ethics, religion or fear? Cell Medicine, 11, 2155179019857661. https://doi.org/10.1177/2155179019857661

Palaniyappan, S. and kumar Sivakumar, N. (2023). Development of crab shell particle reinforced polylactic acid filaments for 3D printing application. Materials Letters, 341, 134257. https://doi.org/10.1016/j.matlet.2023.134257

Phetwarotai, W. and Aht-Ong, D. (2017). Nucleated polylactide blend films with nanoprecipitated calcium carbonate and talc: Preparation, properties, and crystallization kinetics. Journal of Thermal Analysis and Calorimetry, 127, 2367–2381. https://doi.org/10.1007/s10973-016-5802-2

Qu, H.; H. Fu; Z. Han and Y. Sun. (2019). Biomaterials for bone tissue engineering scaffolds: A review. RSC Advances, 9(45), 26252–26262. https://doi.org/10.1039/c9ra05214c

Radwan-Pragłowska, J.; Ł. Janus; M. Piątkowski; D. Bogdał and D. Matysek. (2020). 3D hierarchical, nanostructured chitosan/PLA/HA scaffolds doped with TiO2/Au/Pt NPs with tunable properties for guided bone tissue engineering. Polymers, 12(4), 792. https://doi.org/10.3390/polym12040792

Rajkumar, K.; P. Sirisha and M. R. Sankar. (2014). Tribomechanical and surface topographical investigations of poly methyl methacrylate-seashell particle based biocomposite. Procedia Materials Science, 5, 1248–1257. https://doi.org/10.1016/j.mspro.2014.07.436

Reina, S.; B. Tito; M. Malini; F. Iqrimatien and E. Sa’diyah. (2021). Porosity and compressive strength of PLA-based scaffold coated with hydroxyapatite-gelatin to reconstruct mandibula: A literature review. 1816(1), 012085. https://doi.org/10.1088/1742-6596/1816/1/012085

Saravanan, S.; S. Vimalraj; G. Lakshmanan; A. Jindal; D. Sundaramurthi and J. Bhattacharya. (2019). Chitosan-based biocomposite scaffolds and hydrogels for bone tissue regeneration. Marine-Derived Biomaterials for Tissue Engineering Applications, 413–442.

Sarki, J.; S. Hassan; V. Aigbodion and J. Oghenevweta. (2011). Potential of using coconut shell particle fillers in eco-composite materials. Journal of Alloys and Compounds, 509(5), 2381–2385. https://doi.org/10.1016/j.jallcom.2010.11.025

Shi, J. (2016). Development of functionally graded implant materials in commercial use. Available online at: https://mpra.ub.uni-muenchen.de/id/eprint/76351. Accessed on March 7, 2023.Shi, X.; G. Zhang; T. V. Phuong and A. Lazzeri. (2015). Synergistic effects of nucleating agents and plasticizers on the crystallization behavior of poly (lactic acid). Molecules, 20(1), 1579–1593. https://doi.org/10.3390/molecules20011579

Swaroop, C. and Shukla, M. (2018). Nano-magnesium oxide reinforced polylactic acid biofilms for food packaging applications. International Journal of Biological Macromolecules, 113, 729–736. https://doi.org/10.1016/j.ijbiomac.2018.02.156

Ting, X.; Y. Hongyang; Y. Dongzhi and Y. Zhong-Zhen. (2017). Polylactic Acid Nanofiber Scaffold Decorated with Chitosan Islandlike Topography for Bone Tissue Engineering. 9(25), 21094–21104. https://doi.org/10.1021/acsami.7b01176

Trimeche, M. (2017). Biomaterials for bone regeneration: An overview. Biomater. Tissue Technol, 1, 1–5.

Wang, Z.; Y. Tang; M. Yakufu; L. Li; G. Li; J. Liu and P. Zhang. (2020). Highly Permeable Gelatin/Poly (lactic acid) Fibrous Scaffolds with a Three-Dimensional Spatial Structure for Efficient Cell Infiltration, Mineralization and Bone Regeneration. ACS Applied Bio Materials, 3(10), 6932–6943. https://doi.org/10.1021/acsabm.0c00815

Witte, F. (2010). The history of biodegradable magnesium implants: A review. Acta Biomaterialia, 6(5), 1680–1692. https://doi.org/10.1016/j.actbio.2010.02.028

Yang, F.; X. Ye; J. Zhong; Z. Lin; S. Wu; Y. Hu; W. Zheng; W. Zhou; Y. Wei and X. Dong. (2023). Recycling of waste crab shells into reinforced poly (lactic acid) biocomposites for 3D printing. International Journal of Biological Macromolecules, 234, 122974. https://doi.org/10.1016/j.ijbiomac.2022.12.193

Yasmeen, S.; M. K. Kabiraz; B. Saha; M. Qadir; M. Gafur and S. Masum. (2016). Chromium (VI) ions removal from tannery effluent using chitosan-microcrystalline cellulosecomposite as adsorbent. Int. Res. J. Pure Appl. Chem, 10(4), 1–14. https://doi.org/10.9734/IRJPAC/2016/23315

Zhao, Y.; H. Liang; S. Zhang; S. Qu; Y. Jiang and M. Chen. (2020). Effects of magnesium oxide (MgO) shapes on in vitro and in vivo degradation behaviors of PLA/MgO composites in long term. Polymers, 12(5), 1074. https://doi.org/10.3390/polym12051074.

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Published

2024-06-22