A Novel Acinetobacter sp. Isolated from Oil-contaminated Soil for Microbial Enhanced Oil Recovery

Authors

  • Akeem Olatunde Arinkoola Ladoke Akintola University of Technology, Ogbomoso
  • Lukman Moronkola Rafiu Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Azeez Gbolahan Akinyemi Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Islamiyat Abiodun Oladunjoye Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Ridwan Adetunji Adepoju Department of Pure/Applied biology, Ladoke Akintola University of Technology, Ogbomoso
  • Ebenezer Olujimi Dada Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Solomon Oluyemi Alagbe Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Sunday Babatunde Akinde Department of Microbiology, Ladoke Akintola University of Technology, Ogbomoso
  • Ganiyu Kayode Latinwo Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso
  • Samuel Enahoro Agarry Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso

Keywords:

hyperactive bacteria, thermophilic bacteria, Enhanced oil recovery, Microbial EOR, Biosurfactant production, Bacteria screening, Biomass, metabolites

Abstract

The viscosity of hydrocarbon reservoirs increases with age. So, a significant amount of oil is trapped in the underground reservoirs after recovery using primary and secondary methods. To improve the recovery, tertiary methods have been used. However, there is limited research on Microbial Enhanced Oil Recovery (MEOR) as a tertiary method due to microbes' inability to survive the high-temperature reservoir conditions. Consequently, the goal of this research is the creation of a novel bacteria strain for MEOR. The isolated bacteria colonies were inoculated in a batch fermentation broth, and the biosurfactant produced was screened using the oil displacement, emulsification index, modified drop-collapse test, surface tension, and interfacial tension criteria. The effects of pH (7.2 – 10.52), and salinity (15 – 35 %) at optimum temperature were studied on the selected isolate, which was identified by partial 16 rRNA gene sequence analysis. Gas Chromatography-Mass Spectroscopy was used to characterize the biosurfactants produced. The new isolate reduced heavy oil viscosity by 17% and produced a recovery factor in the range of 13-16.72%. The new bacteria is a thermophile and survived at a temperature of 65 °C, indicating promise for use in MEOR.

References

Aboelkhair, H.; Diaz, P. and Attia, A. (2022). Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery. Journal of Petroleum Science and Engineering, 208(PC), p. 109601.

Al-Wahaibi, Y.M.; Grattoni, C.A. and Muggeridge, A.H. (2006). Drainage and imbibition relative permeabilities at near miscible conditions. Journal of Petroleum Science and Engineering, 53(3–4): 239–253.

Alkan, H.; Mukherjee, S. and Kögler, F. (2020). Reservoir engineering of in-situ MEOR: impact of microbial community. Journal of Petroleum Science and Engineering, 195: 107928.

Almeida, P.F.; Moreira, R. S.; Almeida, R. C. C.; Guimaraes, A. K.; Carvalho, A. S.; Quintella, C.; Esperidia, M.C. A. and Taft, C. A. (2004). Selection and application of microorganisms to improve oil recovery. Engineering in Life Sciences, 4(4): 319–325.

Bachmann, R.T.; Johnson, A.C. and Edyvean, R.G.J. (2014). Biotechnology in the petroleum industry: An overview. International Biodeterioration and Biodegradation, 86: 225–237.

Banat, I.M.; Franzetti, A.; Gandolfi, I.; Bestetti, G.; Martinotti, M. G.; Fracchia, L.; Smyth, T. J. and Marchant, R. (2010). Microbial biosurfactants production, applications and future potential. Applied Microbiology and Biotechnology, 87(2): 427–444.

Berry, J. D.; Neeson, M. J.; Dagastine, R. R.; Chan, D. Y.C. and Tabor, R. F. (2015). Measurement of surface and interfacial tension using pendant drop tensiometry. Journal of Colloid and Interface Science, 454: 226–237.

Bodour, A.A. and Miller-Maier, R.M. (1998). Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms. Journal of Microbiological Methods, 32(3): 273–280.

Boniek, D.; Figueiredo, D.; Pylro, V. S. and Duarte, G. F. (2010). Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica. Extremophiles, 14(5): 475–481.

Campos, J.M.; Stamford, T.L.M. and Sarubbo, L.A. (2014). Production of a bioemulsifier with potential application in the food industry. Applied Biochemistry and Biotechnology, 172(6): 3234–3252.

Chen, S.Y.; Lu, W. B.; Wei, Y. H.; Chen, W. M. and Chang, J.S. (2007). Improved production of biosurfactant with newly isolated Pseudomonas aeruginosa S2. Biotechnology Progress, 23(3): 661–666.

Dastgheib, S. M. M.; Amoozegar, M. A.; Elahi, E.; Asad, S. and Banat, I. M. (2008). Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnology Letters, 30(2): 263–270.

Elazzazy, A.M.; Abdelmoneim, T.S. and Almaghrabi, O.A. (2015). Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi Journal of Biological Sciences, 22(4): 466–475.

Gao, C. (2018). Experiences of microbial enhanced oil recovery in Chinese oil fields. Journal of Petroleum Science and Engineering, 166: 55–62.

Gao, P.; Li, G.; Li, Y.; Li, Y.; Tian, H.; Wang, Y.; Zhou, J. and Ma, T. (2016). An exogenous surfactant-producing Bacillus subtilis facilitates indigenous microbial enhanced oil recovery. Frontiers in Microbiology, 7: 1–14.

Gaol, C.L.; Ganzer, L.; Mukherjee, S. and Alkan, H. (2021). Parameters govern microbial enhanced oil recovery

Aboelkhair, H.; Diaz, P. and Attia, A. (2022). Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery. Journal of Petroleum Science and Engineering, 208(PC), p. 109601.

Al-Wahaibi, Y.M.; Grattoni, C.A. and Muggeridge, A.H. (2006). Drainage and imbibition relative permeabilities at near miscible conditions. Journal of Petroleum Science and Engineering, 53(3–4): 239–253.

Alkan, H.; Mukherjee, S. and Kögler, F. (2020). Reservoir engineering of in-situ MEOR: impact of microbial community. Journal of Petroleum Science and Engineering, 195: 107928.

Almeida, P.F.; Moreira, R. S.; Almeida, R. C. C.; Guimaraes, A. K.; Carvalho, A. S.; Quintella, C.; Esperidia, M.C. A. and Taft, C. A. (2004). Selection and application of microorganisms to improve oil recovery. Engineering in Life Sciences, 4(4): 319–325.

Bachmann, R.T.; Johnson, A.C. and Edyvean, R.G.J. (2014). Biotechnology in the petroleum industry: An overview. International Biodeterioration and Biodegradation, 86: 225–237.

Banat, I.M.; Franzetti, A.; Gandolfi, I.; Bestetti, G.; Martinotti, M. G.; Fracchia, L.; Smyth, T. J. and Marchant, R. (2010). Microbial biosurfactants production, applications and future potential. Applied Microbiology and Biotechnology, 87(2): 427–444.

Berry, J. D.; Neeson, M. J.; Dagastine, R. R.; Chan, D. Y.C. and Tabor, R. F. (2015). Measurement of surface and interfacial tension using pendant drop tensiometry. Journal of Colloid and Interface Science, 454: 226–237.

Bodour, A.A. and Miller-Maier, R.M. (1998). Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms. Journal of Microbiological Methods, 32(3): 273–280.

Boniek, D.; Figueiredo, D.; Pylro, V. S. and Duarte, G. F. (2010). Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica. Extremophiles, 14(5): 475–481.

Campos, J.M.; Stamford, T.L.M. and Sarubbo, L.A. (2014). Production of a bioemulsifier with potential application in the food industry. Applied Biochemistry and Biotechnology, 172(6): 3234–3252.

Chen, S.Y.; Lu, W. B.; Wei, Y. H.; Chen, W. M. and Chang, J.S. (2007). Improved production of biosurfactant with newly isolated Pseudomonas aeruginosa S2. Biotechnology Progress, 23(3): 661–666.

Dastgheib, S. M. M.; Amoozegar, M. A.; Elahi, E.; Asad, S. and Banat, I. M. (2008). Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnology Letters, 30(2): 263–270.

Elazzazy, A.M.; Abdelmoneim, T.S. and Almaghrabi, O.A. (2015). Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi Journal of Biological Sciences, 22(4): 466–475.

Gao, C. (2018). Experiences of microbial enhanced oil recovery in Chinese oil fields. Journal of Petroleum Science and Engineering, 166: 55–62.

Gao, P.; Li, G.; Li, Y.; Li, Y.; Tian, H.; Wang, Y.; Zhou, J. and Ma, T. (2016). An exogenous surfactant-producing Bacillus subtilis facilitates indigenous microbial enhanced oil recovery. Frontiers in Microbiology, 7: 1–14.

Gaol, C.L.; Ganzer, L.; Mukherjee, S. and Alkan, H. (2021). Parameters govern microbial enhanced oil recovery (MEOR) performance in real-structure micromodels. Journal of Petroleum Science and Engineering, 205.

Gudina, E. J.; Pereira, J. F.B.; Costa, R.; Coutinho, J.A.P.; Teixeira, J.A. and Rodrigues, L.R. (2013). Biosurfactant-producing and oil-degrading Bacillus subtilis strains enhance oil recovery in laboratory sand-pack columns. Journal of Hazardous Materials, 261: 106–113.

Hadia, N.J.; Ottenheim, C.; Ng, S.; Hu, Q.; Stubbs, L.P. and Lau, H.C. (2019). Experimental investigation of biosurfactant mixtures of surfactin produced by Bacillus Subtilis for EOR application. Fuel, 251: 789–799.

Ishag, N.; Najib, M. and Hamid, A. (2021). Materials Today : Proceedings The potentials of emulsified modified bitumen ( EMB ) for coating and insulation: An overview. Materials Today: Proceedings.

Jha, N.K.; Ali, M.; Iglauer, S.; Lebedev, M.; Roshan, H.; Barifcani, A.; Sangwai, J.S. and Sarmadivaleh, M. (2019). Wettability Alteration of Quartz Surface by Low-Salinity Surfactant Nano fl uids at High-Pressure and High-Temperature Conditions. Energy & Fuels, 33: 7062–7068.

Kaczorek, E., Olszanowski, A. and Cybulski, Z. (2005). Analysis of surface tension during biodegradation of hydrocarbons. Polish Journal of Environmental Studies, 14(2): 179–183.

Kogler, F.; Mahler, E.; Dopffel, N.; Schulze-Makuch, D.; Borovina, A.; Visser, F.; Herold, A. and Alkan, H. (2021). The Microbial Enhanced Oil Recovery (MEOR) potential of Halanaerobiales under dynamic conditions in different porous media. Journal of Petroleum Science and Engineering, 196.

Kumar, N.J.; Lebedev, M.; Iglauer, S.; Ali, M.; Roshan, H, Barifcani, A.; Sangwai, J.S. and Sarmadivaleh, M. (2020). Pore scale investigation of low salinity surfactant nanofluid injection into oil saturated sandstone via X-ray micro-tomography. Journal of Colloid And Interface Science, 562: 370–380.

Kurniati, T.H.; Rahayu, S.; Sukmawati, D. and Maharani, W. (2019). Screening of biosurfactant producing bacteria from hydrocarbon contaminated soil. Journal of Physics: Conference Series, 1402(5).

Makkar, R.S.; Cameotra, S.S. and Banat, I.M. (2011). Advances in utilization of renewable substrates for biosurfactant production. AMB Express, 1(1): 1–19.

Martzy, R.; Bica-Schröder, K.; Pálvölgyi, A.M.; Kolm, C.; Jakwerth, S.; Kirschner, A. K. T. Sommer, R.; Krska, R.; Mach, R.L.; Farnleitner, A.H. and Reischer, G.H. (2019). Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids. Scientific Reports, 9(1): 1–10.

Morikawa, M. and Hirata, Y. (2000). A study on the structure function relationship of lipopeptide biosurfactants. Biochimica et Biophysica Acta, 1488: 211-218.

Nasiri, M. (2014). The Effect of Oilfield Chemicals on the Surface Tension of Surfactant Systems. Journal of Petroleum Science and Technology, 4(1): 56–66.

Niu, J.; Liu, Q.; Lv, Q. and Peng, B. (2020). Review on microbial enhanced oil recovery: Mechanisms, modeling and field trials. Journal of Petroleum Science and Engineering, 192: 107350.

Okoro, E.E.; Efajemue, E.A.; Sanni, S.E.; Olabode, O.A.; Orodu, O.D. and Ojo, T. (2022). Application of thermotolerant petroleum microbes at reservoir conditions for enhanced oil recovery. Petroleum.

Pannekens, M.; Kroll, L.; Müller, H.; Mbow, F.T. and Meckenstock, R.U. (2019). Oil reservoirs, an exceptional habitat for microorganisms. New Biotechnology, 49: 1–9.

l tension and biosurfactant-bacteria growth. Journal of Petroleum Exploration and Production Technology, 9(3): 2353–2374.

Saravanan, A.; Kumar, P.S.; Vardhan, K.H.; Jeevanantham, S.; Karishma, S.B.; Yaashika, P.R. and Vellaichamy, P. (2020). A review on systematic approach for microbial enhanced oil recovery technologies: Opportunities and challenges. Journal of Cleaner Production, 258: 120777.

Saruni, N.H.; Abdul Razak, S.; Habib, S.; Ahmad, S.A.; Alias, S.A.; Johari, W.L.W.; Mykla, J. and Yasid, N.A. (2019). Comparative Screening Methods for the Detection of Biosurfactant-Producing Capability of Antarctic Hydrocarbon-degrading Pseudomonas sp. Journal of Environmental Microbiology and Toxicology, 7(1): 44–47.

She, Y.H.; Zhang, F.; Xia, J.J.; Kong, S.Q.; Wang, Z.L.; Shu, F.C. and Hu, J.M. (2011). Investigation of biosurfactant-producing indigenous microorganisms that enhance residue oil recovery in an oil reservoir after polymer flooding. Applied Biochemistry and Biotechnology, 163(2): 223–234.

Shibulal, B.; Al-Bahry, S.N.; Al-Wahaibi, Y.M.; Elshafie, A.E.; Al-Bemani, A.S. and Joshi, S.J. (2014). Microbial enhanced heavy oil recovery by the aid of inhabitant spore-forming bacteria: An insight review. The Scientific World Journal, 2014: 1-12.

Soliman, A.A.; El-hoshoudy, A.N. and Attia, A.M. (2020). Assessment of xanthan gum and xanthan-g-silica derivatives as chemical flooding agents and rock wettability modi fiers. IFP Energies nouvelles, 12:1–13.

Soudmand-asli, A.; Ayatollahi, S.S.; Mohabatkar, H.; Zareie, M. and Shariatpanahi, S.F. (2007). The in situ microbial enhanced oil recovery in fractured porous media. Journal of Petroleum Science and Engineering, 58(1–2): 161–172.

US Energy Information Administration (EIA) (2017). International Energy Outlook 2017 Overview. International Energy Outlook 2017, IEO2017(2017): 143.

Wu, B.; Xiu, J.; Yu, L.; Huang, L.; Yi, L. and Ma, Y. (2022). Research advances of microbial enhanced oil recovery. 8: e11424.

Yakimov, M.M.; Amro, M.M.; Bock, M.; Boseker, K.; Fredrickson, H.L.; Kessel, D.G. and Timmis, K.N. (1997). The potential of Bacillus licheniformis strains for in situ enhanced oil recovery. Journal of Petroleum Science and Engineering, 18(1–2): 147–160.

Zhou, H.; Chen, J.; Yang, Z.; Qin, B.; Li, Y. and Kong, X. (2015). Biosurfactant production and characterization of Bacillus sp. ZG0427 isolated from oil-contaminated soil. Annals of Microbiology, 65(4): 2255–2264.

Ziwei, B.; Xiangchun, Z.; Yiying, W.; Lusha, W.; Yifei, W. and Hanning, W. (2021). Review on microbial enhanced oil recovery in China: mechanisms, potential disadvantages, and application of genetic engineering. Energy Sources, Part A: Recovery, Utilization and Environmental Effects, 1–16.

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Published

2024-09-29

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Vol. 21 No. 3 (2024): Volume 21, Issue 3, September 2024

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