Production of Medium Chain Fatty Acids From Ensiled Potato Peels; Effect of Inoculum Type and Kinetic Study
Keywords:
Batch fermentation, chain elongation, electron donors, waste biomassAbstract
Medium chain fatty acids (MCFAs) are fatty acids containing 6 to 12 carbon atoms with a wide range of industrial application. They can be produced by the fermentation of waste biomass through a process called chain elongation (CE). During CE, the type of inoculum used plays a key role in determining the optimal yield of MCFAs. Three different inocula including leachate, rumen fluid and digestate from a biogas reactor were used for the batch fermentation of ensiled potato peels for MCFAs production in this study. Results show that the highest chain elongation was obtained when leachate was used as inoculum with a maximum yield of 57, 4 and 26 g/kgVS for caproic acid, heptanoic acid and caprylic acid respectively. A kinetic study shows that the production of MCFAs from ensiled potato peels was better described by the first-order model than by the modified Gompertz model.
References
References
Allaart, M. T., Stouten, G. R., Sousa, D. Z., & Kleerebezem, R. (2021). Product Inhibition and pH Affect Stoichiometry and Kinetics of Chain Elongating Microbial Communities in Sequencing Batch Bioreactors. Frontiers in Bioengineering and Biotechnology, 9, 693030. https://doi.org/10.3389/fbioe.2021.693030
Atasoy, M., Eyice, O., & Cetecioglu, Z. (2020). A comprehensive study of volatile fatty acids production from batch reactor to anaerobic sequencing batch reactor by using cheese processing wastewater. Bioresource Technology, 311, 123529. https://doi.org/10.1016/j.biortech.2020.123529
Atasoy, M., Eyice, O., Schnürer, A., & Cetecioglu, Z. (2019). Volatile fatty acids production via mixed culture fermentation: Revealing the link between pH, inoculum type and bacterial composition. Bioresource Technology, 292, 121889. https://doi.org/10.1016/j.biortech.2019.121889
Bolaji, I. O., & Dionisi, D. (2017). Acidogenic fermentation of vegetable and salad waste for chemicals production: Effect of pH buffer and retention time. Journal of Environmental Chemical Engineering, 5(6), 5933–5943. https://doi.org/10.1016/j.jece.2017.11.001
Chen, W.-S., Strik, D. P.B.T.B., Buisman, C. J.N., & Kroeze, C. (2017). Production of Caproic Acid from Mixed Organic Waste: An Environmental Life Cycle Perspective. Environmental Science & Technology, 51(12), 7159–7168. https://doi.org/10.1021/acs.est.6b06220
Coelho, M. M. H., Morais, N. W. S., Pereira, E. L., Leitão, R. C., & Santos, A. B. d. (2020). Potential assessment and kinetic modeling of carboxylic acids production using dairy wastewater as substrate. Biochemical Engineering Journal, 156, 107502. https://doi.org/10.1016/j.bej.2020.107502
Fang, W., Zhang, P., Zhang, T., Requeson, D. C., & Poser, M. (2019). Upgrading volatile fatty acids production through anaerobic co-fermentation of mushroom residue and sewage sludge: Performance evaluation and kinetic analysis. Journal of Environmental Management, 241, 612–618. https://doi.org/10.1016/j.jenvman.2019.02.052
Fogler, H. S. (2016). Elements of Chemical Reaction Engineering (5th ed.). New York: Prentice Hall.
Groof, V. de, Coma, M., Arnot, T., Leak, D. J., & Lanham, A. B. (2019). Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation. Molecules, 24(3). https://doi.org/10.3390/molecules24030398
Iglesias-Iglesias, R., Fernandez-Feal, M. M. d. C., Kennes, C., & Veiga, M. C. (2021). Valorization of agro-industrial wastes to produce volatile fatty acids: Combined effect of substrate/inoculum ratio and initial alkalinity. Environmental Technology, 42(25), 3889–3899. https://doi.org/10.1080/09593330.2020.1743370
Jankowska, E., Chwialkowska, J., Stodolny, M., & Oleskowicz-Popiel, P. (2017). Volatile fatty acids production during mixed culture fermentation – The impact of substrate complexity and pH. Chemical Engineering Journal, 326, 901–910. https://doi.org/10.1016/j.cej.2017.06.021
Leeuw, K. D. de, Buisman, C. J.N., & Strik, D. P.B.T.B. (2019). Branched Medium Chain Fatty Acids: Iso-Caproate Formation from Iso-Butyrate Broadens the Product Spectrum for Microbial Chain Elongation. Environmental Science & Technology, 53(13), 7704–7713. https://doi.org/10.1021/acs.est.8b07256
Lu, Y., Zhang, Q., Wang, X., Zhou, X., & Zhu, J. (2020). Effect of pH on volatile fatty acid production from anaerobic digestion of potato peel waste. Bioresource Technology, 316, 123851. https://doi.org/10.1016/j.biortech.2020.123851
Ma, H., Wu, W., Yu, Z., Zhao, J., Fu, P., Xia, C., Gao, M. (2022). Medium-chain fatty acid production from Chinese liquor brewing yellow water by electro-fermentation: Division of fermentation process and segmented electrical stimulation. Bioresource Technology, 360, 127510. https://doi.org/10.1016/j.biortech.2022.127510
Morais, N. W. S., Coelho, M. M. H., Silva, A. d. S. e., Pereira, E. L., Leitão, R. C., & Santos, A. B. d. (2020). Kinetic modeling of anaerobic carboxylic acid production from swine wastewater. Bioresource Technology, 297, 122520. https://doi.org/10.1016/j.biortech.2019.122520
Perimenis, A., Nicolay, T., Leclercq, M., & Gerin, P. A. (2018). Comparison of the acidogenic and methanogenic potential of agroindustrial residues. Waste Management, 72, 178–185. https://doi.org/10.1016/j.wasman.2017.11.033
Ren, W., Wu, Q., Deng, L., Hu, Y., Guo, W., & Ren, N. (2022). Simultaneous medium chain fatty acids production and process carbon emissions reduction in a continuous-flow reactor: Re-understanding of carbon flow distribution. Environmental Research, 212, 113294. https://doi.org/10.1016/j.envres.2022.113294
Saadoun, L., Campitelli, A., Kannengiesser, J., Stanojkovski, D., Fels, A. E. A. E., Mandi, L., & Ouazzani, N. (2022). Acidogenic digestion of organic municipal solid waste in a pilot scale reactor: Effect of waste ratio and leachate recirculation and dilution on hydrolysis and medium chain fatty acid production. Bioresource Technology Reports, 17, 100872. https://doi.org/10.1016/j.biteb.2021.100872
Sample, G. (2022). Ensiling of Potato Peel Waste for Biomethane Production; An Evaluation of Kinetic Parameters. Nigerian Journal of Engineering Science and Technology Research, 8(1), 42–50.
Sarkar, O., Rova, U., Christakopoulos, P., & Matsakas, L. (2021). Ethanol addition promotes elongation of short-chain fatty acids to medium-chain fatty acids using brewery spent grains as substrate. Journal of Environmental Chemical Engineering, 9(5), 105990. https://doi.org/10.1016/j.jece.2021.105990
Silva, A. d. S. e., Ferreira, T. J. T., Morais, N. W. S., Pereira, E. L., & Santos, A. B. d. (2021). S/X ratio impacts the profile and kinetics of carboxylic acids production from the acidogenic fermentation of dairy wastewater. Environmental Pollution, 287, 117605. https://doi.org/10.1016/j.envpol.2021.117605
Undiandeye, J., Gallegos, D., Lenz, J., Nelles, M., & Stinner, W. (2022). Effect of Novel Aspergillus and Neurospora Species-Based Additive on Ensiling Parameters and Biomethane Potential of Sugar Beet Leaves. Applied Sciences, 12(5). https://doi.org/10.3390/app12052684
Undiandeye, J., Gallegos, D., Sträuber, H., Nelles, M., & Stinner, W. (2022). Ensiling parameters in vertical columns and multiple kinetic models evaluation of biomethane potential of ensiled sugar beet leaves. Biofuels, 13(8), 995–1005. https://doi.org/10.1080/17597269.2022.2059964
Villegas-Rodríguez, S., & Buitrón, G. (2021). Performance of native open cultures (winery effluents, ruminal fluid, anaerobic sludge and digestate) for medium-chain carboxylic acid production using ethanol and acetate. Journal of Water Process Engineering, 40, 101784. https://doi.org/10.1016/j.jwpe.2020.101784
Wu, Q., Feng, X., Chen, Y., Liu, M., & Bao, X. (2021). Continuous medium chain carboxylic acids production from excess sludge by granular chain-elongation process. Journal of Hazardous Materials, 402, 123471. https://doi.org/10.1016/j.jhazmat.2020.123471
Wu, S.-L., Sun, J., Chen, X., Wei, W., Song, L., Dai, X., & Ni, B.-J. (2020). Unveiling the mechanisms of medium-chain fatty acid production from waste activated sludge alkaline fermentation liquor through physiological, thermodynamic and metagenomic investigations. Water Research, 169, 115218. https://doi.org/10.1016/j.watres.2019.115218
Xu, J., Bian, B., Angenent, L. T., & Saikaly, P. E. (2021). Long-Term Continuous Extraction of Medium-Chain Carboxylates by Pertraction With Submerged Hollow-Fiber Membranes. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.726946
Yebouet, O. F., Campitelli, A., Amoikon, S. L. T., Kannengiesser, J., Stanojkovski, D., Mrukwia, T., . . . Djeni, T. N.’D. (2021). Assessment of the diversity and abundance of bacterial population and its correlation with medium chain fatty acids production from fermentation of two leachate qualities. Bioresource Technology Reports, 16, 100840. https://doi.org/10.1016/j.biteb.2021.100840
Zhang, W., Wang, S., Yin, F., Cao, Q., Lian, T., Zhang, H., . . . Dong, H. (2022). Medium-chain carboxylates production from co-fermentation of swine manure and corn stalk silage via lactic acid: Without external electron donors. Chemical Engineering Journal, 439, 135751. https://doi.org/10.1016/j.cej.2022.135751
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